Background: Rhodiola rosea is a herb that has been used in traditional medicine for
several years, and LF is a class of lipoproteins derived from the fish Trachurus sp. (LF-T), which
exhibits known anti-inflammatory activity.
Objective: Investigating the anti-aging effect of Rhodiola specific bioactive fractions cluster in
combination with LF-T (R-L compound) in H2O2 mediated oxidative stress-induced human amnion
derived epithelial cell line - FL cells as normal human cell line.
Methods: FL cells were treated with H2O2 to induce cellular aging, followed by treatment of the RL
compound to study its anti-aging characteristics. Based on the proliferation rate, 0.05% and 0.1%
concentration of R-L compound was determined using MTT assay. Anti-aging and anti-oxidant assays,
ABTS, DPPH, Hyaluronidase activity Nitric Oxide, Lipid Peroxidase, and Superoxide Dismutase
were performed. qPCR for anti-aging genes and matrix metalloproteinase genes were analyzed.
Results: FL cells treated with R-L compound exhibited increased proliferation rate and free-radical
reduction. Decreased Hyaluronidase enzyme activity and regulation of genes such as SIRT1,
KLOTHO, SERPINA 6, MMP 9, and MMP 2 expression depicted the anti-aging role of the R-L
compound. Chemometric profiling of the R-L compound revealed that aromatic compounds and
unsaturated fatty acids along with their derivatives, were present predominantly, which might have
attributed to the potent oxidative stress impeded aging activity.
Conclusion: Specific Bioactive Fractions of Rhodiola in combination with LF-T obtained from
Trachurus sp. involve in the regulation of aging genes and might be a novel approach to prevent
the cells from oxidative stress damage and also it might avert the aging of cells.