Mesenchymal Stromal Cell-derived Exosomes Attenuate Experimental Pulmonary Arterial Hypertension

Author(s): LiLi Ge, Wen Jiang, Shanshan Zhang, Jue Wang, Qian Xin, Chao Sun, Kailin Li, Tonggang Qi, Yun Luan*

Journal Name: Current Pharmaceutical Biotechnology

Volume 22 , Issue 12 , 2021


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Abstract:

Background: Pulmonary arterial Hypertension (PH) is a chronic disease that ultimately progresses to right ventricular failure and death. Until now, there is still a lack of effective treatment applied. The purpose of the present study was to observe the protective effect of Mesenchymal Stromal Cell-Derived Exosomes (MSC-EXO) against experimental Pulmonary arterial Hypertension (PH) and right ventricular failure.

Methods: All the experimental rats received an intraperitoneal injection of 50 mg/kg monocrotaline to induce PH model. Three weeks after the model was successfully established, the cell Culture Media (CM) or MSC-EXO derived from human umbilical cord was administered daily via the tail vein. All animals were randomly divided into 4 groups: Control (saline-treated), MCT-PH, MCT-CM and MCT-EXO groups. Post-operation, hemodynamic data and index of right ventricular hypertrophy (RVHI) were recorded to evaluate the inhibition of MSC-EXO on MCT-induced PH. Histology, immunohistochemistry and western blot were used to analyze the effect of MSC-EXO against vascular remodeling and further reveal the mechanism.

Results: In the present study, our results showed that MSC-EXO administration could significantly reduce the Right Ventricular Systolic Pressure (RVSP) and RVHI, suppress the pulmonary vascular remodeling and The Endothelial-Mesenchymal Transition (EndMT) process.

Conclusion: Our results provided the firm information for a new method in the treatment of PH; the mechanism may be related to the inhibition of vascular remodeling and EndMT.

Keywords: Pulmonary arterial hypertension, pulmonary vascular remodeling, exosomes, MSC-EXO, right ventricular hypertrophy, EndMT.

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Article Details

VOLUME: 22
ISSUE: 12
Year: 2021
Published on: 30 December, 2020
Page: [1654 - 1662]
Pages: 9
DOI: 10.2174/1389201022666201231113127
Price: $65

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