Analysis of Chemical Composition by GC-MS and in-vitro Antidiabetic Activity of Azadirachta indica Leaves Collected from Erbil, Iraq

Author(s): Javed Ahamad*, Subasini Uthirapathy, Esra T. Anwer, Muath Sh. Mohammed Ameen, Faiq H.S. Hussain

Journal Name: Current Traditional Medicine

Volume 7 , Issue 4 , 2021


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Abstract:

Background: Azadirachta indica(Neem) is an important medicinal plant that is traditionally known for its insecticidal, and antimicrobial properties.

Objective: The objective of present study was to evaluate the in-vitro antidiabetic activity and characterize the chemical constituents of Neem extract.

Materials and Methods: The chemical constituents in ethyl acetate extract of the Neem leaves were determined by GC-MS and the in-vitro antidiabetic activity of Neem extract was evaluated via the inhibition of α-amylase and α-glucosidase enzymes.

Results: The ethyl acetate extract of Neem yielded 49 chemical compounds and these constituents amounting to 97.07% of total Neem leaf extract composition. The major chemical components of the Neem were octadecanoic acid (7.17%), propyl tetracosyl ether (5.35%), 1-decanol, 2-octyl (5.01%), hexadecane (4.70%), tricosane (4.73%), octadecane, 1-chloro (4.65%), and linolenic acid (4.35%). The Neem extract produces dose dependent-inhibition of α-amylase and α-glucosidase enzymes ranging from 21.67±2.81 to 75.82±3.44% and 17.04±4.43 to 58.44±1.21% for concentration 31.25 to 1000 μg/mL, respectively. The IC50 values for Neem extract and acarbose were 165.53±2.75 and 91.04±2.16μg/mL, respectively against the α-amylase enzyme. The IC50 values for Neem extract and acarbose were 210.08±2.16 and 120.05±2.16 μg/mL, respectively against the α-glucosidase enzyme.

Conclusion: The study findings suggested that Neem may be used as drug candidate in the management of post prandial hyperglycemia.

Keywords: Azadirachta indica, Neem, GC-MS, α-amylase, α-glucosidase, PPHG, diabetes.

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Article Details

VOLUME: 7
ISSUE: 4
Year: 2021
Published on: 30 December, 2020
Page: [613 - 619]
Pages: 7
DOI: 10.2174/2215083807999201230123003
Price: $95

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