Objective: Achillea fragrantissima L. (Asteraceae) is a traditionally used medicinal herb in the rural communities
Methods: The present study evaluated the efficacy of the ethanol extract of this species on angiogenesis in both, ex vivo using rat aortic ring assay and in vivo using rat excision wound model.
Results: In concentrations of 50 and 100 µg/ml, the ethanol extract showed angiogenic stimulatory effect and significantly
increased length of capillary protrusions around aorta rings of about 60% in comparison to those of untreated aorta rings. In
MCF-7 cells, the ethanol extract of A. fragrantissima stimulates the production of VEGF in a dose-dependent manner. 1%
and 5% of ethanol extract of A. fragrantissima containing vaseline based ointment was applied on rat excision wounds for
six days and was found to be effective in wound healing and maturation of the scar. Both preparations resulted in better
wound healing when compared to the untreated control group and vaseline-treated group. This effect was comparable to that
induced by MEBO, the positive control.
Conclusion: The results indicate that A. fragrantissima has a pro-angiogenic effect, which may act through the VEGF signaling pathway.