The utilization of N-glycan profiling recently gained high importance in
fundamental biomedical and applied clinical research. However, for the time being, no
glycan biomarker has been approved for clinical diagnosis by the regulatory agencies
due to the lack of verifications on large patient cohorts and suitable analytical
technologies. In this paper, the effect of human blood sample handling was studied prior
to N-glycosylation profiling by capillary electrophoresis, coupled with high sensitivity
fluorescence detection. Special attention was paid to the preservation of sialylated
structures because of their important clinical - biological relevance. Our results
suggested that it is adequate to refrigerate and store the collected total blood samples
prior to analysis to obtain unbiased results. Furthermore, we report on the good practice
of serum sample handling in order to prevent decomposition of the sialylated structures.
Our findings may promote procedure standardization and easier clinical translation of
diagnostic N-glycosylation profiling in molecular medicinal applications.
Keywords: Blood sampling, storage temperature, storage time, preanalytical processing, N-glycome, capillary
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