Objectives: The issue of food-additive-toxicity causing several health hazards needs to be therapeutically
managed in an immediate effect. Alloxan, a food additive, used for whitening and shining of flour, is capable of inducing
genotoxicity, diabetes, and associated mitochondrial dysfunction. Therefore, to explore a non-toxic, phyto-based compound
which could delay the onset of diabetes and prevent the multitude of damage associated therein, Chlorophyllin (CHL), was
selected for our study having been reported to exhibit anti-cancer, anti-diabetes, anti-inflammatory responses. Therefore, the
objectives of the present study aimed to evaluate the protective role of CHL in controlling genotoxicity, glucose imbalance,
and associated cytochrome c mediated mitochondrial signaling dysfunction against food-additive-induced genotoxicity,
diabetic state, and its complexities in mice model in vivo.
Method: Mice were pre-treated with CHL through oral gavage before they were exposed to alloxan. Diabetic markers, antioxidant enzyme profile, chromosomal study, mitochondrial functioning factors, and expression of proteins were checked
against food-additive injected mice.
Results: The results revealed that CHL pre-treatment could delay the onset of diabetes, restrict alloxan-induced elevation of
blood glucose, reduce DNA-damage and chromosomal aberration, optimize enzymatic profile (glucokinase, pyruvate,
insulin), and modulates protein expression (insulin, IRS1, IRS2, GLUT2). Further, CHL-pre-treatment could stabilize
mitochondrial-membrane-potential, intracellular calcium ion, ATP/ADP ratio, ATPase activity thereby maintaining
optimum functioning of cytochrome-c, bcl2, and caspase3 mitochondrial protein.
Conclusion: Therefore, the present study reports for the first time, the screening of phyto-based bioactive CHL for
preventing/limiting the extent of food-additive-induced genotoxicity and mitochondrial dysfunction and serve as an
advanced therapeutic tool in the management of diabetes.