Compositional Analysis and Antioxidant Activities of Thymus pubescens Essential Oil from Iran

(E-pub Ahead of Print)

Author(s): Bahman Nickavar*, Elaheh Malekitabar

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

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Background: The genus Thymus is one of the well-known members of the Lamiaceae, which comprises about 215 species. It is a polymorphic genus in this family. Therefore, possible differences in chemical composition among polymorphs may result in differences in their biological activities.

Objective: This study was designed to specify chemical composition in order to assess antioxidant activities and to characterize active constituents of essential oil from Thymus pubescens.

Methods: Essential oil was collected by hydrodistillation and was analyzed using GC-MS and GC-FID. Antioxidant activities were evaluated by ABTS radical cation scavenging and β-carotene bleaching inhibition methods. Detection and identification of antioxidant components were performed by TLC-bioautography assay using ABTS•+ reagent.

Results: GC analyses indicated the presence of 39 components, accounting for 96.64% of total constituents, with thymol (38.67%), γ-terpinene (7.46%), and p-cymene (5.54%) as the main components. The IC50 values for antioxidant activities were equal to 1.15 (1.05 – 1.25) μg/mL and 0.45 (0.33 – 0.62) μg/mL for ABTS•+ scavenging and β-carotene bleaching inhibition tests, respectively. TLC-bioautograpgy of oil resulted in identification of thymol as the major antioxidant compound.

Conclusion: Essential oil exhibited very potent and significant antioxidant activities correlated well with increasing dose in vitro.

Keywords: Antioxidant activity, bioautography, chemical composition, essential oil, Lamiaceae, Thymus pubescens.

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Article Details

Published on: 29 December, 2020
(E-pub Ahead of Print)
DOI: 10.2174/1386207323666201230091042
Price: $95

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