Objective: The purpose of this study was to explore the mechanism of the miR-375/XPR1 axis in esophageal
squamous cell carcinoma (ESCC) and provide a new idea for targeted therapy of ESCC.
Methods: Differentially expressed genes in GEO and TCGA databases were analyzed by bioinformatics. The expression
levels of miR-375 and XPR1 mRNA were detected by qRT-PCR. Protein expression of XPR1 was detected by western blot.
Bioinformatics analysis and dual luciferase assay were conducted to confirm the targeting relationship between miR-375
and XPR1. The viability, proliferation, migration and invasion of cells in each treatment group were detected by CCK-8,
colony formation, wound healing and Transwell assays.
Results: Significantly down-regulated miR-375 and remarkably up-regulated XPR1 were observed in ESCC tissue and
cells. Overexpression of miR-375 inhibited proliferation, invasion and migration of ESCC cells, and greatly reduced the
promoting effect of XPR1 overexpression on cell proliferation, migration and invasion. Dual luciferase assay confirmed that
miR-375 targeted and inhibited XPR1 expression in ESCC.
Conclusion: These results demonstrate the regulatory role of the miR-375/XPR1 axis in ESCC cells and provide a new
potential target for the precise treatment of patients with ESCC.