Objective: The aim of this work is to identify and purify bioactive compounds from
70℅ aqueous methanolic extract (AME) of B. spectabilis leaves collected in Cairo, Egypt and assessed their rheumatoid arthritis activity.
Methods: The methanolic extract of B. spectabilis leaves was fractionated and subjected to different chromatographic techniques to isolate pure new compounds which were identified by one dimensional and two dimensional nuclear magnetic resonance (NMR) spectroscopic analyses and
mass spectrometric methods. The isolated compounds were evaluated for their anti-inflammatory
activity on adjuvant induced chronic rheumatoid arthritis.
Results & Discussion: Seven bioactive compounds were purified and identified for the first time
from the methanolic extract of Bougainvillea spectabilis leaves; secologanin dimethyl acetal,
α- and β-amyrin, α- and β-amyrin acetate, Kaempferol and kaempferol-3-O-rhamnoside. The previously mentioned compounds had a significant effect against rheumatoid arthritis.
Conclusion: The seven compounds isolated from the methanolic extract of B. spectabilis leaves
showed strong anti-rheumatoid arthritis activity.
Keywords: B. spectabilis, rheumatoid arthritis, secologanin dimethyl acetal, α- amyrin, β-amyrin, α-amyrin acetate, β-amyrin
acetate, kaempferol and kaempferol-3-O-rhamnoside.
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