Background:: Fibromyalgia syndrome (FMS) is a chronic disease characterized by
widespread body pain, weakness in certain parts of the body (critical points), low pain tolerance,
sleep disturbances, and fatigue. This syndrome is considered rare in Jordan.
Objectives: The research aimed to find out the association of the angiotensin converting enzyme,
methylenetetrahydrofolate reductase, and vitamin D receptor (ACE, MHFTR, and VDR, respectively)
genotypes with FMS among Jordanian patients.
Methods: This work included 22 FM patients and 22 healthy individuals of Jordanian Arabic origin.
The ACE rs4646994, MTHFR rs1801133, and VDR rs2228570 genotypes were determined using
polymerase chain reaction (PCR) followed by restriction fragment length polymorphism.
Results: No associations between ACE rs4646994, MTHFR rs1801133, and VDR rs2228570 with
the vulnerability of a person for the development of FMS were found. However, we found an association
between the ACE rs4646994 genotype and restless leg among FM patients.
Conclusion: Based on the result from this study, it appears that the ACE rs4646994 genotype is associated
with restless leg among FMS patients of Jordanian origin. Further clinical investigations
with larger sample sizes are required to confirm these findings and understand the molecular mechanism
of ACE rs4646994 genetic variant in the restless leg syndrome among FM patients.