Background: Bronchial Asthma (BA) is a complex heterogeneous disease with a number
of molecular immunopathological mechanisms underlying airway inflammation, hyperreactivity,
and bronchial remodeling. MicroRNAs are important regulators in the pathogenesis and progression
of chronic respiratory diseases, including BA.
Objective: The aim of the study is to investigate the level of expression of cell-free circulating
miR-19b-3p and miR-320c in the blood plasma by comparing their plasma levels with IL-4 in the
moderate BA patients and control group.
Methods: The level of expression miR-19b-3p and miR-320c were evaluated by qRT-PCR using
the comparative threshold cycle (Ct) method. U6 small nuclear RNA was taken as an endogenous
control. The content of IL - 4 in blood plasma was determined by using ELISA.
Results: miR-19b-3p and miR-320c were significantly dysregulated in moderate asthmatic patients
in comparison with control group. The area under the ROC curve of miR-19b-3p and miR-320c
showed 0.8088 (95% CI 0.6925 to 0.9251, P value =0.0001) and 0,9048 (95% CI 0,7792 to 1,000,
P value <0,0001), respectively. BA patients showed a considerably positive correlation between
the expression level of microRNA-320c and IL-4 levels.
Conclusion: These cell-free circulating microRNAs are probably deregulated in other inflammatory/
pathological diseases, so they could be useful to understand the molecular pathogenesis of BA
or to investigate the “inflammatory reaction” in this disease.