Background: Lately, the employment of nano-carriers has been known as an optimistic
means of drug and vaccine delivery.
Objective: Nano vaccines are a novel tier of vaccines that can develop humoral and cellular immune
responses and can be introduced as a practical and secure nano vaccine candidate for the prevention
of diseases. The purpose of this study was to accomplish and use biodegradable nano-carriers for the
synthesis of pentavalent vaccine and immunogenicity evaluation in the animal models. The PLGA
nanoparticle was prepared and modified with chitosan nanoparticles. Nano-carrier PLGA-chitosan
was loaded by the DTP-HepB-Hib antigens and confirmed by DLS, SEM, TEM and FT-IR, then
in vitro loading and release were evaluated. Toxicity was assessed by the MTT method in the
Hec293 cells. Antigenicity evaluation and histopathological study were conducted by injection of
new nano pentavalent vaccines in the BALB/c mice and the immune response was measured in the
serum samples through an indirect ELISA method.
Results: Conclusions drawn from the current study exhibited the plausible ability of nano-carrier to
deliver the vaccine. A notable increase was shown in total IgG and IgM antibodies examined in
mice vaccinated with new nano vaccines. The histopathological study in the treated mice showed no
toxicity in the vital organs of mice.
Conclusion: The engineered vaccine delivery system showed the ability to induce robust immune
responses and also suitability features of the PLGA-chitosan as a promising carrier to improve vaccine