Background: Carissa spinarum Linn has been used as a traditional medicine to treat various
ailments, including snakebite. It is found in India, Ceylon and Thailand.
Objective: The study was performed to determine the inhibiting potential of Carissa spinarum
methanolic leaf extract on the pharmacological effects of Viper venom.
Methods: The dose-dependent enzymatic studies, pharmacological and in vivo studies were conducted
using standard methods.
Results: It neutralized toxic enzymes in a dose-dependent manner with concentrations ranging from
53.3 –1190.4 μg/mL, inhibited lysis of fibrinogen at 1:8 (venom: extract, w/w), and increased the
procoagulant activity and lecithin lysis at 1:25 (venom: extract, w/w). The extract neutralized the
LD50 of venom in mice and embryos, reduced haemorrhage, myotoxicity and edema induced by the
venom in mice.
Conclusion: The observed results confirm that the leaf extract possesses adequate phytochemicals
that could neutralize the toxic properties of the venom.