Curcumin-containing Silver Nanoparticles Prevent Carbon Tetrachloride-Induced Hepatotoxicity in Mice

(E-pub Ahead of Print)

Author(s): Hossam Ebaid*, Mohamed Habila, Iftekhar Hassan, Jameel Al-Tamimi, Mohamed S. Omar, Ahmed Rady, Ibrahim M Alhazza

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research


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Abstract:

Background: Hepatotoxicity remains an important clinical challenge. Hepatotoxicity observed in response to toxins and hazardous chemicals may be alleviated by delivery of the curcumin in silver nanoparticles (AgNPs-curcumin). In this study, we examined the impact of AgNPs-curcumin in a mouse model of carbon tetrachloride (CCl4)-induced hepatic injury.

Methods: Male C57BL/6 mice were divided into three groups (n=8 per group). Mice in group 1 were treated with vehicle control alone, while mice in Group 2 received a single intraperitoneal injection of 1 ml/kg CCl4 in liquid paraffin (1:1 v/v). Mice in group 3 were treated with 2.5 mg/kg AgNPs-curcumin twice per week for three weeks after the CCl4 challenge.

Results: Administration of CCL4 resulted in oxidative dysregulation, including significant reductions in reduced glutathione and concomitant elevations in the level of malondialdehyde (MDA). CCL4 challenge also resulted in elevated levels of serum aspartate transaminase (AST) and alanine transaminase (ALT); these findings were associated with the destruction of hepatic tissues. Treatment with AgNPs-curcumin prevented oxidative imbalance, hepatic dysfunction, and tissue destruction. A comet assay revealed that CCl4 challenge resulted in significant DNA damage as documented by a 70% increase in nuclear DNA tail-length; treatment with AgNPs-curcumin inhibited the CCL4-mediated increase in nuclear DNA tail-length by 34%.

Conclusion: Administration of AgNPs-curcumin resulted in significant antioxidant activity in vivo. This agent has the potential to prevent the hepatic tissue destruction and DNA damage that results from direct exposure to CCL4.

Keywords: Carbon tetrachloride, Oxidative stress, Hepatotoxicity, liver function, Silver nanoparticles, CurcuminCarbon tetrachloride, Oxidative stress, Hepatotoxicity, liver function, Silver nanoparticles, Curcumin

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Article Details

(E-pub Ahead of Print)
DOI: 10.2174/1386207323666201211100830
Price: $95

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