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Combinatorial Chemistry & High Throughput Screening

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ISSN (Print): 1386-2073
ISSN (Online): 1875-5402

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Research Article

A Fusion Method Based on Alignment Software with SNP and Indel Detection Methods

Author(s): Guobing Chen, Chao Tang, Jun Qi, Ying Wang* and Xiaolong Shi*

Volume 25, Issue 3, 2022

Published on: 11 December, 2020

Page: [519 - 527] Pages: 9

DOI: 10.2174/1386207323666201211095018

Price: $65

Abstract

Background: With the advent of the second-generation sequencing technology, the discovery of sequence alignment and sequence variation is a long-standing challenge

Methods: A method based on general alignment software, SNP, and Indel software tools was proposed in this paper. By comparing the advantages of traditional alignment software, we can produce the best alignment.

Results: SNP and Indel detection tools fusion research found that different depth of sequencing effect is different. When the sequence depth reaches a certain value, one of the softwares is selected for testing

Conclusion: Finally, the experimental verification shows that SNP and Indel have certain advantages in comparing the effects of the fusion method.

Keywords: Second-generation sequencing, SNP, indel, fusion research, software tools, sequence alignment

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