Aim: Apart from the modifiable risk factors, genetic factors are believed to influence the
outcome of Coronary Artery Diseases (CAD). Under the genetic factors, miRNA polymorphisms,
namely Hsa-miR-146a-5p (rs2910164) have become an important tool to study the mechanism that
underlies the pathogenesis of this disease. Therefore, we investigated the association of miR-146a
gene variations with susceptibility of coronary artery diseases.
Methodology: This study was conducted on 100 CAD patients and 117 matched healthy individuals.
Genotyping of the Hsa-miR-146a-5p C>G gene variation was performed by using Amplification
Refractory Mutation System PCR method (ARMS-PCR).
Results: The distribution of Hsa-miR-146a-5p rs2910164 C>G genotypes observed between patients
and controls was significantly different (P=0.048). Moreover, the frequency of G allele (fG)
was found to be significantly higher among patients than in controls (0.36 vs. 0.25). Our findings
showed that the Hsa-miR-146a-5p C>G variant was associated with an increased risk of CAD in codominant
inheritance model CC vs. CG genotype (OR = 1.84, 95% CI, 1.02-3.31; p=0.040) and
(OR = 3.18, 95% CI, 1.02-9.9; p=0.045) for CC vs. GG genotype in dominant inheritance model.
Whereas the G allele significantly increased the risk of coronary artery disease (OR =1,81, 95% CI,
1.18-2.78; p=0.006) compared to C allele. Taken together, these results demonstrated that
miR-146a/rs2910164 is associated with susceptibility to coronary artery disease, providing novel
insights into the genetic etiology and underlying biology of coronary artery disease.
Conclusion: Our findings indicated that Hsa-miR-146a-5p rs2910164 GG genotype and G allele
are associated with increased susceptibility to Coronary Artery Disease. A larger sample size can
be the key to progress in establishing the genetic co-relation of miRNA gene polymorphisms and