Aim and Objective: Lung cancer is the most commonly occurring cancer, which
contributes to the majority of death caused by cancer, where non-small-cell lung cancer (NSCLC)
accounts for approximately 85% of lung cancer. To treat NSCLC, STAT3 has been identified as a
target with therapeutic potential. The neobavaisoflavone (NBIF) is one of the flavonoids of
traditional Chinese medicine Psoralea corylifolial.
Materials and Methods: Human NSCLC cell lines, PC-9, H460, and A549, were applied to
determine NBIF’s anti-proliferative effects through cell viability and colony formation detection.
The effect of NBIF on cell apoptosis was determined through flow cytometry-based assay. Western
blotting was used in this study to confirm the levels of P-STAT3, Bcl-2, and Bax, which are
Results: It was observed that NBIF could decrease the cell viability and its migration and induce
apoptosis in human NSCLC cell lines dose-dependently. Levels of P-STAT3, as well as the
downstream signals of the STAT3 pathway, were downregulated, suggesting that the tumorsuppression
effects of NBIF might be related to the inhibition of STAT3 signaling. Furthermore,
NBIF could contribute to the upregulation of BAX and downregulation of BCL2.
Conclusion: NBIF might perform the anti-NSCLC efficacy as a result of the inhibition of the
STAT3 pathway. Besides, our work suggests that NBIF could provide therapeutic alternatives for