Aims: In the present study we aimed to investigate neuroprotective effects of Nardostachys
jatamansi against BSO induced anxiety in mice.
Background: Oxidative stress is implicated in nervous system impairment and development of
anxiety-like disorders. There is an immediate surge for identification of herbal supplements to treat
oxidative stress mediated anxiety and neurodegener-ation.
Objective: In present study, we analyzed the metabolites present in 70 % ethanolic extract of Nardostachys
jatamansi (NJE) by LC-ESI-MS/MS and RP-HPLC. Oxidative stress was induced using
BSO (300 mg/kg) for 7 days after pretreatment with Nardostachys jatamansi extract (250 mg/kg)
followed by assessing anxiety levels in mice using behavioural indices and biochemical parameters
as well as western blot of two important biomarkers linking oxidative stress with anxiety viz gly-oxalase
1 and glutathione reductase.
Methods: LC–ESI-MS/MS analysis aided in identification of the major metabolites present in NJE.
RP-HPLC analysis for neurotransmitter content, behavioural tests for anxiety analysis, oxidative
stress markers by biochemical analysis, and west-ern blot analysis for oxidative stress markers
Results: LC–ESI-MS/MS analysis aided in identification of the major metabolites present in NJE.
A total of 15 metabolites were identified. RP-HPLC analysis for serotonin and melatonin content
revealed an enriched melatonin content 72.19 ± 1.6 μg/g, however, serotonin could not be detected.
The anxiolytic tests employed showed that BSO-induced oxidative stress for 7 days caused a significant
decrement in time spent in open arm of EPM, in exploratory behavior in OFT and light compartment
of LDB and also in a number of licks and shocks accepted in VCT. NJE further decreased
oxidative stress mediated markers in serum viz cortisol, lipid peroxides and protein carbonyls in
brain and improved the antioxidant status of brain (GPx, GR, GST, SOD, CAT, and ABTS). NJE
also mitigated levels of choline and glutamate in brain. Moreover, NJE in-creased brain GABA and
monoamine levels thereby effectively overcoming the anxiety-like effects of BSO.
Conclusion: These results clearly suggest that OS mediates anxiety and NJE could be an effective
remedy in treating oxi-dative stress mediated anxiety and neuropsychiatric disorders.