Identification of Major Compounds and α-Amylase and α-Glucosidase Inhibitory Activity of Rhizome of Musa balbisiana Colla: An in-vitro and in-silico Study

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Author(s): Ananta Swargiary*, Manita Daimari

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research


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Abstract:

Background: α-Amylase and α-glucosidase inhibitors are widely used to suppress postprandial glycemia in the treatment of type 2 diabetes.

Objectives: To evaluate the metallic content, major phytoconstituents, and α-amylase and α-glucosidase inhibitory activity of Musa balbisiana rhizome using in-vitro and in-silico methods.

Materials and Methods: Heavy metal content was detected by AAS following standard protocol. Major phytochemicals of the plant were analysed by GC-MS technique. Enzyme inhibition study was carried out by UV/VIS spectrophotometric methods. The druglikeness and bioavailability properties of major compounds were carried out using computer-aided tools – SwissADME and ADMElab. Docking and visualization were performed in AutoDock vina and Discovery studio tools.

Results: The study found that the fruits of M. balbisiana contain negligible amount toxic elements. GC-MS analysis showed five major compounds from the rhizome of M. balbisiana. In-vitro enzyme assays revealed strong α-amylase and αglucosidase inhibitory property of the plant. All the five compounds were predicted to have druglikeness property with high cell membrane permeability and bioavailability. The compounds were also predicted to have low to moderate toxicity property. The Docking study showed strong binding affinities of plant compounds with α-amylase and α-glucosidase. Out of five compounds, C5 showed best binding affinity with active pockets of α-amylase and α-glucosidase.

Conclusion: The present in-vitro and in-silico study suggests the antihyperglycemic property of the rhizome of Musa balbisiana and possible candidate for therapeutic antidiabetic agent(s).

Keywords: Musa balbisiana, rhizome, α-amylase, α-glucosidase, docking, ADMET

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(E-pub Ahead of Print)
DOI: 10.2174/1386207323666201124144332
Price: $95

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