Background: Chlorophytum comosum, popularly known as Spider Ivy, is used as a medicinal plant in
traditional Chinese medicine, however, its detailed chemical composition and biological activity are yet
Objective: To carry out the phytochemical investigation on different parts of Chlorophytum comosum using GCMS/
LC-ESI-MS and evaluation of its antioxidant, hemolytic and antiproliferative potential on breast cancer
(MCF-7), lung cancer (A549, H1299) and normal lung (L-132) cell lines.
Methods: Chemical constituents from aqueous roots and leaves extracts were identified using LC-ESI-MS/GCMS.
The identified compounds were annotated based on the match of mass spectra with the literature using NIST
14 and METLIN databases. Antioxidant activity was studied using DPPH, FRAP and TPC assays. The
antiproliferative effects of ethanolic roots and leaves extracts of Chlorophytum comosum were measured by MTT
assay on breast cancer (MCF-7), lung cancer (A549 & H1299) and normal lung (L-132) cell lines. The toxicity
studies of the extracts were carried out using Hemolysis assay.
Results: GC-MS analysis identified 34 metabolites in roots and 17 from leaves, while 17 compounds from roots
and 7 from leaves were detected by LC-ESI-MS. Significant antiproliferative effects were observed on the A549
and MCF-7 cancer cell lines with IC50 values ranging from 56.86 μg/ml to 68.68 μg/ml while no marked
response was observed against normal cell line L-132.
Conclusion: Our study represents the first report on the detailed chemical composition and antiproliferative
potential of Chlorophytum comosum against lung and breast cancer cell lines.