Aim and Objective: This study aimed at investigating the gastro-protective effects of
Algerian Sahara (Sidr) honey from Apis mellifera intermissa against HCl/Ethanol-induced gastric
ulcers in rats.
Materials and Methods: Total phenolic content, antioxidant activity and phenolic compounds were
determined. Then, three groups of rats (control, HCl/ Ethanol-induced ulcer, and orally
administered honey) were used for the determination of gastro-protective effect of Sidr honey.
Results: Total phenolic content, total flavonoid content, and DPPH activity of the honey sample
were determined as 47.35±3.35 mg GAE/ 100 g, 2.13±0.17 mg QE/ 100 g, and 229.24±0.02
mg/mL, respectively. Oral pretreatment of rats with honey (1.2 g/Kg body weight orally at an
interval of 2 days) protected gastric mucosa against HCl/Ethanol-induced damage by decreasing
ulcer score, the volume and acidity of gastric juice and increasing pH.
Conclusion: These results were confirmed by the histological assessment, which demonstrated a
significant gastro-protective activity of Saharian (Sidr) honey against HCl/Ethanol-induced
stomach ulcer. Plasma tumor necrosis factor-α, IL-6 and PGE2 were also measured. Sahara honey
significantly decreased the plasma TNF-α, PGE2, and IL-6 concentrations.