Background: The use of nanoparticles has markedly increased in biomedical sciences.
The silver nanoparticles (AgNPs) have been investigated for their applicability to deliver
chemotherapeutic/antibacterial agents to treat cancer or infections disease. However, the existing
chemical and physical methods of synthesizing AgNPs are considered inefficient, expensive and toxic.
Methods: Natural products have emerged as viable candidates for nanoparticle production,
including the use of Terfezia boudieri (T. boudieri), a member of the edible truffle family.
Accordingly, our goal was to synthesize AgNPs using an aqueous extract of T. boudieri (green
synthesized AgNPs). Since certain infectious agents are linked to cancer, we investigated their
potential as anti-cancer and antibacterial agents.
Results: The synthesis of AgNPs was confirmed by the presence of an absorption peak at 450nm
by spectroscopy. The physico-chemical properties of green synthesized AgNPs were analyzed by
UV-Vis, FT-IR, XRD, SEM, and TEM. In addition, their potential to inhibit cancer cell
(proliferation and the growth of infectious bacteria were investigated.
Conclusion: The size of nanoparticles ranged between 20-30nm. They exerted significant
cytotoxicity and bactericidal effects in a concentration and time-dependent manner compared to T.
boudieri extract alone. Interestingly, the synthesis of smaller AgNPs was correlated with longer
synthesis time and enhanced cytotoxic and bactericidal properties.