Background: In clinical treatment, it is difficult to carry out effective bone tissue transplantation
and anti-inflammatory treatment at the same time due to bone defects and osteomyelitis
where the tissue is contaminated or infected. As a downstream target of TNF-α, NF-κB has an inhibition
effect on the proliferation and differentiation of cells surrounding the lesion. As a negative effect,
it leads to a reduction in bone growth and development.
Methods: In this study, the small molecule NBD polypeptide and bone conduction matrix Sr-CaS
are microspheres, formed to prepare Sr-CaS, NBD drug-loaded sustained-release microspheres in
order to achieve a Sr-CaS/NBD peptide drug-loaded sustained release microsphere scaffold material
(SP-Sr-CaS/NBD). We prepared the microspheres and optimized the production process to obtain
particles with stable morphological properties and sustained release properties.
Result: In vitro experiments demonstrated that SP-Sr-CaS/NBD could reduce TNF-α-induced cell
growth inhibition, caspase-3 activity and NF-κB transcriptional activation as the function of continuous
NBD peptide dosing regimen.
Conclusion: Also, the introduction of the Sr-CaS matrix potentiates microspheres to promote cell
proliferation and provides a basis to become a promising 3D bone scaffold material in the future.