Background: Propolis is a natural resinous material produced by honeybees. The
biological activity and phenolic profile of propolis were largely studied all over the world.
However, only a few investigations have been carried out on Algerian and Turkish propolis. The
aim of the present study was to compare the phenolic content, antioxidant, and antibacterial
activity of propolis samples collected from different localities of Algeria and Turkey.
Methods: Propolis extracts were performed using maceration in ethanol 80%. Total phenolic and
flavonoid contents were determined. Antioxidant and antibacterial activities were evaluated using
FRAP assay and the MIC was determined against four bacterial strains (S. aureus ATCC 25923, E.
coli ATCC 25922, P. aeruginosa ATCC 27853 and K. pneumonia).
Results: TP varied from 19.51 ± 0.86 to 219.66 ± 1.23 mg GAE/g. Whereas, TF varied from 5.27±
0.07 to 74.57 ± 1.03 QE/g. All samples showed good ferric reducing antioxidant power ranging
from 267.30 ± 4.77 to 2387.30 ± 44.15 μmol Trolox eq./g. All Algerian propolis samples displayed
a more pronounced activity against S. aureus ATCC 25923 with MIC values ranging from 0,04 ±
0.00 mg/mL to 0.30±0.06 mg/Ml, with an activity 30 times more powerful than Anatolian propolis.
While, Anatolian propolis samples were most active against P. aeruginosa ATCC 27853 with MIC
values ranging from 0.20±0.00 mg/mL to 0.60±0.00 mg/Ml, with an activity 5 to 10 times more
powerful than Algerian propolis.
Conclusion: Algerian and Anatolian propolis possessed considerable phenolic and flavonoids
contents. In addition, they exhibited interesting antioxidant and antibacterial activities. Our
findings suggest that both propolis could be useful in the food and pharmaceutical industries.