Abstract
Background: Establishing R, S-enantiomer (S-KT and R-KT) chiral resolution and determination methods for KT are of great significance.
Objective: This study aimed to establish a high-performance liquid chromatography (HPLC) method for the resolution and determination of ketorolac tromethamine (KT) enantiomers.
Methods: A CHIRALPAK AGP column (0.4 × 10 cm, 5 μm) was used with 10 mmol/L ammonium acetate (pH 5.5) and isopropanol (97:3) as the mobile phase. The detection wavelength was 324 nm, the flow rate was 1.0 mL/min, the column temperature was 25°C, and the injection volume was 5 μL.
Results: The resolution between S-KT and R-KT was 2.8. S-KT and R-KT demonstrated a good linear relationship in the range of 3-60 μg/mL (r > 0.999). The average recoveries of S-KT and RKT were 99.2% and 99.8%, with relative standard deviations of 2.0% and 2.4%, respectively.
Conclusion: The established method can be used for the resolution and determination of S-KT and R-KT.
Keywords: Ketorolac tromethamine, enantiomers, HPLC, chiral resolution, determination, respiratory depression.
Current Pharmaceutical Analysis
Title:Chiral Resolution and Content Determination of Ketorolac Tromethamine using High-performance Liquid Chromatography
Volume: 17 Issue: 10
Author(s): Lei Zheng*, Jing Yang, Yu-yao Guan, Lei Zhang, Chao Song*Xiao-li Zhang*
Affiliation:
- School of Medicine and Pharmacy, Ocean University of China, 5 Yushan Road, Qingdao, 266003,China
- Departement of Pharmacy, Shandong Provincial Third Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250031,China
- Departement of Pharmacy, Shandong Provincial Third Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250031,China
Keywords: Ketorolac tromethamine, enantiomers, HPLC, chiral resolution, determination, respiratory depression.
Abstract:
Background: Establishing R, S-enantiomer (S-KT and R-KT) chiral resolution and determination methods for KT are of great significance.
Objective: This study aimed to establish a high-performance liquid chromatography (HPLC) method for the resolution and determination of ketorolac tromethamine (KT) enantiomers.
Methods: A CHIRALPAK AGP column (0.4 × 10 cm, 5 μm) was used with 10 mmol/L ammonium acetate (pH 5.5) and isopropanol (97:3) as the mobile phase. The detection wavelength was 324 nm, the flow rate was 1.0 mL/min, the column temperature was 25°C, and the injection volume was 5 μL.
Results: The resolution between S-KT and R-KT was 2.8. S-KT and R-KT demonstrated a good linear relationship in the range of 3-60 μg/mL (r > 0.999). The average recoveries of S-KT and RKT were 99.2% and 99.8%, with relative standard deviations of 2.0% and 2.4%, respectively.
Conclusion: The established method can be used for the resolution and determination of S-KT and R-KT.
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Cite this article as:
Zheng Lei *, Yang Jing , Guan Yu-yao , Zhang Lei , Song Chao *, Zhang Xiao-li *, Chiral Resolution and Content Determination of Ketorolac Tromethamine using High-performance Liquid Chromatography, Current Pharmaceutical Analysis 2021; 17 (10) . https://dx.doi.org/10.2174/1573412917999201110203024
DOI https://dx.doi.org/10.2174/1573412917999201110203024 |
Print ISSN 1573-4129 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-676X |
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