Biomarker Analysis Based Chemoprofiling of Polyherbal Ayurvedic Formulation Containing Vitis vinifera L. by Validated UPLC-MS/MS Method

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Author(s): Prerna Goel, Vidhu Aeri*, Rikeshwer Prasad Dewangan, Rehan Abdur Rub

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

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Background: Drakshasava is one of the commercial Ayurvedic medicine from India, prepared from grapes and spices. It is believed to address health imbalances and claimed to be beneficial for weakness bleeding disorders and various inflammatory diseases. It reported pharmacological activities such as diuretic, cardioprotective and antimicrobial. Being a polyherbal mixture, it faces challenges in its standardization and quality control.

Objective The aim of the present study is to develop a validated UPLC-MS/MS method for simultaneous quantification of ten polyphenolic biomarkers in Drakshasava. It explores the effect of Vitis vinifera L. and additional herbs on fermentation with respect to bioactive compounds through successive addition method.

Method: The MS methods were optimized in multiple-reaction monitoring (MRM) mode with ESI while chromatographic separation was achieved on an Acquity UPLC BEH C18 column using both isocratic and gradient elution in water and acetonitrile containing 0.1% formic acid.

Results: The developed method was validated as per ICH-Q2B guidelines and found to be within the assay variability limits. Gallic acid was found to be the most abundant marker in all the samples followed by resveratrol. The content of all the markers has found to be increased significantly post fermentation, compared to decoction except kaempferol. The successive addition of prashpeka drvya (minor herbs) in the formulation, show variability at different stages, with respect to the selected markers and does not infer major changes in the chemical profiling of final product.

Conclusion: The developed method was found rapid, accurate, reliable and highly sensitive for the simultaneous quantification of selected biomarkers in Drakshasava. The research is the first chemometric report in standardization of Drakshasava by validated UPLC-MS/MS method. It may prove to be useful tool for development of new phyto-pharmaceutical drug and further quality control of other poly herbal formulations.

Keywords: Ayurvedic formulation, Biomarkers, Chemoprofiling, Drakshasava., Polyherbal, UPLC-MS/MS, Validation, Vitis vinifera L.

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(E-pub Ahead of Print)
DOI: 10.2174/1386207323666201110151817
Price: $95

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