The pyrazolines give the reactions of aliphatic derivatives, resembling unsaturated compounds
in their behavior towards permanganate and nascent hydrogen. This nucleus has been associated
with various biological activities, including inflammatory action. Thiazolinone is a heterocyclic
compound that contains both sulfur and nitrogen atom with a carbonyl group in their structure.
Thiazolinone and their derivatives have attracted continuing interest because of their various biological
activities, such as anti-inflammatory, antimicrobial, anti-proliferative, antiviral, anticonvulsant, etc.
The aim of the research was to club pyrazoline nucleus with thiazolinone in order to have a significant
anti-inflammatory activity. The synthesized compounds were chemically characterized for the establishment
of their chemical structures and to evaluate it as an anti-inflammatory agent.
In the present work, eight derivatives of substituted pyrazoline (PT1-PT8) were synthesized by a threestep
reaction. The compounds were subjected to spectral analysis by Infrared, Mass, and Nuclear magnetic
resonance spectroscopy and elemental analysis data. All the synthesized derivatives were evaluated
for their in vivo anti-inflammatory activity. The synthesized derivatives were evaluated for their affinity
towards target COX-1 and COX-2, using indomethacin as the reference compound molecular
docking visualization through AutoDock Vina.
Compounds PT-1, PT-3, PT-4, and PT-8 exhibited significant anti-inflammatory activity at 3rd hour,
being 50.7%, 54.3%, 52.3%, and 57%, respectively, closer to that of the standard drug indomethacin
(61.9%). From selected anti-inflammatory targets, the synthesized derivatives exhibited better interaction
with COX-1 and COX-2 receptor, where indomethacin showed a docking score of -6.5 kJ/mol,
compound PT-1 exhibited the highest docking score of -9.1 kJ/mol for COX-1 and compound PT-8
had a docking score of 9.4 kJ/mol for COX-2.
It was concluded that synthesized derivatives have more interaction with COX-2 receptors in comparison
to the COX-1 receptors because the docking score with COX-2 receptors was very good. It is concluded
that the synthesized derivatives (PT-1 to PT-8) are potent COX-2 inhibitors.