Functional Characterization and Structural Modelling of Peptidoglycan Degrading β-N-acetyl-glucosaminidase from a Dental Isolate of Serratia marcescens

Author(s): Aditi Rathee, Anil Panwar, Seema Kumari, Sanjay Chhibber, Ashok Kumar*

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

Volume 24 , Issue 9 , 2021

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Introduction: Enzymatic degradation of peptidoglycan, a structural cell wall component of Gram-positive bacteria, has attracted considerable attention being a specific target for many known antibiotics.

Methods: Peptidoglycan hydrolases are involved in bacterial lysis through peptidoglycan degradation. β-N-acetyl-glucosaminidase, a peptidoglycan hydrolase, acts on O-glycosidic bonds formed by N-acetylglucosamine and N-acetyl muramic acid residues of peptidoglycan. Aim of present study was to study the action of β -N-acetylglucosaminidase, on methicillin-resistant Staphylococcus aureus (MRSA) and other Gram-negative bacteria.

Results: We investigated its dynamic behaviour using molecular dynamics simulation and observed that serine and alanine residues are involved in catalytic reaction in addition to aspartic acid, histidine, lysine and arginine residues. When simulated in its bound state, the RMSD values were found lesser than crystal form in the time stamp of 1000 picoseconds revealing its stability. Structure remained stably folded over 1000 picoseconds without undergoing any major change further confirming the stability of complex.

Conclusion: It can be concluded that enzymes belonging to this category can serve as a tool in eradicating Gram-positive pathogens and associated infections.

Keywords: Peptidoglycan hydrolase, beta-N-acetyl-glucosaminidase, docking, molecular dynamic simulations, drug-resistant strains, drugs.

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Article Details

Year: 2021
Published on: 03 November, 2020
Page: [1514 - 1526]
Pages: 13
DOI: 10.2174/1386207323999201103204234
Price: $65

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