Aim and Objective: Diabetes mellitus is associated with inflammation and increased
oxidative stress. In the current study, we aimed to investigate the relationship between type 2
diabetes mellitus (T2DM) and serum pro-oxidant-antioxidant balance (PAB) in a large populationbased
Methods: In this cross-sectional study, 7888 individuals were recruited as part of the Mashhad
Stroke and Heart Atherosclerotic Disorders (MASHAD) cohort study. Participants were divided
into three groups based on their serum PAB values (levels < 36.4, 36.4-82.6 and > 82.6 HK).
Serum PAB values were measured using a colorimetric method and enzyme-linked immune
sorbent assay (ELISA).
Results: Serum PAB in subjects with and without diabetes was reported 76.85 ± 61.07 HK and
69.51 ± 55.50 HK. In subjects with a serum PAB > 82.6 HK the risk of T2DM was 1.2 fold higher
in comparison to subjects with a serum PAB < 36.4 HK (OR: 1.26, 95% CI: 1.09 – 1.47, P-value:
0.002). This association remained significant after adjustment for confounding factors in
multivariable analysis (OR: 1.19, 95% CI: 1.02 – 1.38, P-value: 0.027).
Conclusion: Increased pro-oxidant levels may be a major complication of T2DM in our study
subjects and PAB could be an indicator of higher oxidative stress in T2DM patients from