Introduction: Hydatidiosis is a serious parasitic disease in humans and livestock, worldwide.
Echinococcus granulosus shows notable genetic variation among intermediate hosts. Several
genotypes of the worm have been reported from different parts of Iran, but no information on the
parasite genotypes status in the study region is available. The current study investigated the presence
of different genotypes of E. granulosus in the livestock of Qazvin, Iran, by sequencing the mitochondrial
Methodology: One hundred twenty E. granulosus isolates, including 30 from goats, 40 from cattle
and 50 from sheep, were collected from the slaughterhouses in Qazvin province. Mitochondrial
Cox1 gene region was amplified by PCR and 30 isolates were sequenced. Phylogenetic analysis
was performed by using the MEGA 7.0 software. Morphological analysis was performed on rostellar
hook length of protoscoleces.
Results: All isolates were identified as E. granulosus sensu stricto (G1-G3 complex) among 17%
of the isolates clarified as G3 genotypes. G1 was the predominant genotype among the specimens.
No significant difference between the rostellar hooks measurements of different genotypes was observed.
Conclusion: Our findings confirmed the presence of E. granulosus sensu stricto in the region, although
further studies are required to determine the haplotype diversity of E. granulosus using different
mitochondrial and nuclear genes.