Background: Putranjiva roxburghii Wall is traditionally known to cure many pathological
conditions including epilepsy.
Objective: The present study is aimed at determining bioactive compounds in ethanolic extract of
Putranjiva roxburghii test extract (PRTE) seeds by GCMS analysis and to assess its antiepileptic
potential using various experimental models of epilepsy.
Methods: The ethanolic extract of seeds of Putranjiva roxburghii was subjected to GC-MS analysis
to detect the bioactive phytoconstituents. Acute oral toxicity of the extract was performed
using OCED guideline 420. Pentylenetetrazol (PTZ) kindling model of epilepsy and Maximal
electroshock epilepsy (MES) model of epilepsy were used to determine anti-epileptic potential.
Results: The GC-MS analysis of the extract revealed the presence of 20 phytoconstituents. The
major phytoconstituents included n-Propyl heptyl ether (25.25%), 5-Ethyl hydantoin (8%), octadec-
9-enoic acid (16.25%) and 1, 2-Benzene dicarboxylic acid (11.86%). The PRTE (50 mg/kg
and 100 mg/kg) afforded a significant and dose-dependent protection against PTZ-induced kindling
epilepsy and MES induced epilepsy (p<0.001 and p<0.01).
Conclusion: Based on the above findings, it is evident that Putranjiva roxburghii seeds contain
biologically active compounds. It can also be concluded that the extract possesses anti-epileptic