Purification of Bacteriocin from Lactobacillus plantarum KY449289 and its Role in the Preservation of Mixed Fruit Juice

Author(s): Ranga Mounika, Jannatul F. Siddique, Nivetha Anbalagan, Subathra C. Devi, Mohanasrinivasan V*

Journal Name: Current Bioactive Compounds

Volume 17 , Issue 7 , 2021

Article ID: e010621187202
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Graphical Abstract:


Background: The aim of this study is to isolate, extract and purify the antibacterial protein from Lactobacillus plantarum KY449289 and to assess its bio preservative potential.

Objective: The present study was conducted to evaluate the laxative activity of Hydroethanolic Pericarp Extract of sapindus emarginatus (HESE) in animal models.

Methods: Bacteriocins are antimicrobial peptides synthesized ribosomally which can be used as bio-preservative reducing the risk of chemical preservative’s effect and also replacing the thermal treatments. This study aims to isolate, purify the antibacterial protein from Lactobacillus plantarum KY449289 and to assess its bio preservative potential. In this study, twenty strains were isolated from a yogurt sample and preliminary characterization was carried out, such as morphological, biochemical, and molecular levels as a preliminary assay.

Results: The characterized strains were assessed for the antibacterial activity by well diffusion assay, whereas all twenty strains were shown to have a antibacterial activity against foodborne pathogens, among which two strains VITMM04 and VITMM05 shows the highest zone of inhibition of 15 mm and 10 mm respectively against Listeria monocytogenes. The potent isolate VITMM05 solely showed a broad spectrum antibacterial activity against the pathogens. These strains were further subjected to optimization, purification, and application studies. A high increase in the inhibition zone was recorded with an optimum temperature of 35°C and its activity was found to be stable up to 85°C, optimum pH 6, and 5 mM of EDTA as an enhancer surfactant. On subjected to RP-HPLC, the purified sample showed a distinct peak at 2.192 min corresponding to the peak at 2.192 min for standard bacteriocin.

Conclusion: The six-fold purified bacteriocin was effective in controlling 87% of the microbial population with 20mm zone of inhibiton against the Listeria monocytogenes and this shows the effective role of purified bacteriocin in inhibiting bacterial growth in a greater extent.

Keywords: Bacteriocin, Lactic Acid Bacteria (LAB), purification, biopreservative, mixed fruit juice, microbial population.

Gulahmadov SG, Abdullaeva NF, Guseinova NF, et al. Isolation and characterization of bacteriocin-like inhibitory substances from lactic acid bacteria isolated from Azerbaijan cheeses. Appl Biochem Microbiol 2009; 45(3): 266-71.
Sharma N, Kapoor G, Neopaney B. Characterization of a new bacteriocin produced from a novel isolated strain of Bacillus lentus NG121. Antonie van Leeuwenhoek 2006; 89(3-4): 337-43.
[http://dx.doi.org/10.1007/s10482-005-9036-8] [PMID: 16779629]
Tagg JR, Dajani AS, Wannamaker LW. Bacteriocins of gram-positive bacteria. Bacteriol Rev 1976; 40(3): 722-56.
[http://dx.doi.org/10.1128/MMBR.40.3.722-756.1976] [PMID: 791239]
Stoyanova LG, Egorov NS, Fedorova GB, Katrukha GS, Netrusov AI. A comparison of the properties of bacteriocins formed by Lactococcus lactis subsp. lactis strains of diverse origin. Appl Biochem Microbiol 2007; 43(6): 604-10.
Greco M, Mazzette R, De Santis EP, Corona A, Cosseddu AM. Evolution and identification of lactic acid bacteria isolated during the ripening of Sardinian sausages. Meat Sci 2005; 69(4): 733-9.
[http://dx.doi.org/10.1016/j.meatsci.2004.11.004] [PMID: 22063151]
Klaenhammer TR. Bacteriocins of lactic acid bacteria. Biochimie 1988; 70(3): 337-49.
[http://dx.doi.org/10.1016/0300-9084(88)90206-4] [PMID: 3139051]
McKay LL, Baldwin KA. Applications for biotechnology: present and future improvements in lactic acid bacteria. FEMS Microbiol Rev 1990; 7(1-2): 3-14.
[http://dx.doi.org/10.1111/j.1574-6968.1990.tb04876.x] [PMID: 2271224]
Tenea GN, Yépez L. Bioactive compounds of lactic acid bacteria. Case study: Evaluation of antimicrobial activity of bacteriocin-producing lactobacilli isolated from native ecological niches of Ecuador. Probiotics and prebiotics in Human Nutrition and Health 2016; 149-67.
Vickers NJ. Animal Communication: When I’m Calling You, Will You Answer Too? Curr Biol 2017; 27(14): R713-5.
[http://dx.doi.org/10.1016/j.cub.2017.05.064] [PMID: 28743020]
Todorov SD. Bacteriocins from Lactobacillus plantarum - production, genetic organization and mode of action: produção, organização genética e modo de ação. Braz J Microbiol 2009; 40(2): 209-21.
[http://dx.doi.org/10.1590/S1517-83822009000200001] [PMID: 24031346]
Mathara JM, Schillinger U, Kutima PM, Mbugua SK, Holzapfel WH. Isolation, identification and characterisation of the dominant microorganisms of kule naoto: the Maasai traditional fermented milk in Kenya. Int J Food Microbiol 2004; 94(3): 269-78.
[http://dx.doi.org/10.1016/j.ijfoodmicro.2004.01.008] [PMID: 15246238]
Corr SC, Li Y, Riedel CU, O’Toole PW, Hill C, Gahan CG. Bacteriocin production as a mechanism for the antiinfective activity of Lactobacillus salivarius UCC118. Proc Natl Acad Sci USA 2007; 104(18): 7617-21.
[http://dx.doi.org/10.1073/pnas.0700440104] [PMID: 17456596]
Mataragas M, Metaxopoulos J, Galiotou M, Drosinos EH. Influence of pH and temperature on growth and bacteriocin production by Leuconostoc mesenteroides L124 and Lactobacillus curvatus L442. Meat Sci 2003; 64(3): 265-71.
[http://dx.doi.org/10.1016/S0309-1740(02)00188-2] [PMID: 22063012]
Muriana PM, Klaenhammer TR. Purification and partial characterization of lactacin F, a bacteriocin produced by Lactobacillus acidophilus 11088. Appl Environ Microbiol 1991; 57(1): 114-21.
[http://dx.doi.org/10.1128/AEM.57.1.114-121.1991] [PMID: 1903624]
Ogunbanwo ST, Sanni AI, Onilude AA. Characterization of bacteriocin produced by Lactobacillus plantarum F1 and Lactobacillus brevis OG1. Afr J Biotechnol 2003; 2(8): 219-27.
Mørtvedt CI, Nissen-Meyer J, Sletten K, Nes IF. Purification and amino acid sequence of lactocin S, a bacteriocin produced by Lactobacillus sake L45. Appl Environ Microbiol 1991; 57(6): 1829-34.
[http://dx.doi.org/10.1128/AEM.57.6.1829-1834.1991] [PMID: 1872611]
Lyon WJ, Glatz BA. Partial purification and characterization of a bacteriocin produced by Propionibacterium thoenii. Appl Environ Microbiol 1991; 57(3): 701-6.
[http://dx.doi.org/10.1128/AEM.57.3.701-706.1991] [PMID: 16348436]
Venema K, Chikindas ML, Seegers J, et al. Rapid and efficient purification method for small, hydrophobic, cationic bacteriocins: purification of lactococcin B and pediocin PA-1. Appl Environ Microbiol 1997; 63(1): 305-9.
[http://dx.doi.org/10.1128/AEM.63.1.305-309.1997] [PMID: 16535495]
Holck A, Axelsson L, Birkeland SE, Aukrust T, Blom H. Purification and amino acid sequence of sakacin A, a bacteriocin from Lactobacillus sake Lb706. J Gen Microbiol 1992; 138(12): 2715-20.
[http://dx.doi.org/10.1099/00221287-138-12-2715] [PMID: 1487735]
Martínez Viedma P, Abriouel H, Sobrino López A, et al. Effect of enterocin AS-48 in combination with high-intensity pulsed-electric field treatment against the spoilage bacterium Lactobacillus diolivorans in apple juice. Food Microbiol 2009; 26(5): 491-6.
[http://dx.doi.org/10.1016/j.fm.2009.03.001] [PMID: 19465245]
Ołdak A, Zielińska D, Rzepkowska A, Kołożyn-Krajewska D. Comparison of antibacterial activity of Lactobacillus plantarum strains isolated from two different kinds of regional cheeses from Poland: Oscypek and Korycinski Cheese. BioMed research international 2017.
Wang Y, Qin Y, Xie Q, Zhang Y, Hu J, Li P. Purification and characterization of plantaricin LPL-1, a novel class IIa bacteriocin produced by Lactobacillus plantarum LPL-1 isolated from fermented fish. Front Microbiol 2018; 9: 2276.
[http://dx.doi.org/10.3389/fmicb.2018.02276] [PMID: 30323792]
Ali WS, Musleh RM. Purification and characterization of plantacirin vgw8, a bacteriocin produced by Lactobacillus plantarum VGW8. J Biol 2015; 5(1): 147-52.
Zahid M, Mustafa BE. Determination of biopreservative effects of bacteriocins isolated from lactic acid producing bacteria against food spoiling fungi. Int J Curr Microbiol Appl Sci 2015; 4(2): 88-96.
Čanak I, Markov K, Melvan E, et al. Isolation and characterisation of L. plantarum O1 producer of plantaricin as potential starter culture for the biopreservation of aquatic food products. Food Technol Biotechnol 2018; 56(4): 581-9.
[http://dx.doi.org/10.17113/ftb.] [PMID: 30923455]
Okereke HC, Achi OK, Ekwenye UN, Orji FA. Antimicrobial properties of probiotic bacteria from various sources. Afr J Biotechnol 2012; 11(39): 9416-21.
Vaithilingam M, Chandrasekaran S, Mehra A, et al. Fermentation of beet juice using lactic acid bacteria and its cytotoxic activity against human liver cancer cell lines HepG2. Curr Bioact Compd 2016; 12(4): 258-63.
Silva CCG, Silva SPM, Ribeiro SC. Application of bacteriocins and protective cultures in dairy food preservation. Front Microbiol 2018; 9: 594.
[http://dx.doi.org/10.3389/fmicb.2018.00594] [PMID: 29686652]
Tenea GN, Barrigas A. The efficacy of bacteriocin-containing cell-free supernatant from Lactobacillus plantarum Cys5-4 to control pathogenic bacteria growth in artisanal beverages. Int Food Res J 2018; 25(5)
Pilevar Z, Hosseini H, Beikzadeh S, Khanniri E, Alizadeh AM. Application of Bacteriocins in Meat and Meat Products: An Update. Curr Nutr Food Sci 2020; 16(2): 120-33.

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Year: 2021
Published on: 26 October, 2020
Article ID: e010621187202
Pages: 8
DOI: 10.2174/1573407216999201026193645
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