Metabolic Profile of Dendrobine in Rats Determined by Ultra-high-performance Liquid Chromatography/Quadrupole Time-of-flight Mass Spectrometry

Author(s): Jun-qi Bai, Qian-xiang Guo, Jing Zhang, Juan Huang, Wen Xu, Lu Gong, He Su, Yu-bing Luo, Jian-hua Li, Xiao-hui Qiu, Zhi-hai Huang*

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

Volume 24 , Issue 9 , 2021


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Abstract:

Aims: Dendrobine is a major alkaloid present mainly in dendrobium nobile Lindl. It has been reported to have analgesic, antipyretic, lower heart rate and blood pressure and other pharmacologic activities. Despite its critical pharmacological function, its metabolite profiling is still unclear.

Methods: In this study, the in vivo metabolite profiling of dendrobine in rats was investigated using ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC/Q-TOF-MS). The metabolites were predicted using MetabolitePilotTM software with a mass defect filter (MDF) technique. These predicted metabolites were further analyzed by MS2 spectra and compared with the detailed fragmentation pathway of the dendrobine standard and literature data.

Results: Total of 59 metabolites were identified for the first time in rat plasma and urine after oral administration of dendrobine. Demethylated, dehydrogenated, hydroxylated, ketonizated and glucuronide were the major metabolic pathways.

Conclusion: This research provides scientific and reliable support for full understanding of the metabolic fate of dendrobine in vivo.

Keywords: Dendrobine, metabolites, UPLC-Q-TOF-MS, urine, mass defect filter, UHPLC.

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Article Details

VOLUME: 24
ISSUE: 9
Year: 2021
Published on: 23 October, 2020
Page: [1364 - 1376]
Pages: 13
DOI: 10.2174/1386207323666201023115744
Price: $65

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