Aims: Dendrobine is a major alkaloid present mainly in dendrobium nobile Lindl. It has
been reported to have analgesic, antipyretic, lower heart rate and blood pressure and other pharmacologic
activities. Despite its critical pharmacological function, its metabolite profiling is still unclear.
Methods: In this study, the in vivo metabolite profiling of dendrobine in rats was investigated using
ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass
spectrometry (UPLC/Q-TOF-MS). The metabolites were predicted using MetabolitePilotTM software
with a mass defect filter (MDF) technique. These predicted metabolites were further analyzed
by MS2 spectra and compared with the detailed fragmentation pathway of the dendrobine standard
and literature data.
Results: Total of 59 metabolites were identified for the first time in rat plasma and urine after oral
administration of dendrobine. Demethylated, dehydrogenated, hydroxylated, ketonizated and glucuronide
were the major metabolic pathways.
Conclusion: This research provides scientific and reliable support for full understanding of the
metabolic fate of dendrobine in vivo.