The complex immune system is involved in multiple pathological processes. Single-cell
RNA sequencing (scRNA-seq) is able to analyze complex cell mixtures correct to a single cell and
single molecule, thus is qualified to analyze immune reactions in several diseases. In recent years,
scRNA-seq has been applied in many researching fields and has presented many innovative results. In
this review, we intend to provide an overview of single-cell RNA sequencing applications in immunology
and a prospect of future directions.
Keywords: Single-cell RNA sequencing, immunology, cancer, autoimmune disease, transplantation, tumors.
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