Background: Shrimp waste, as an important source of natural carotenoids, is produced
in large quantities in the seafood processing industry. One of the important characteristics of
carotenoids is their ability to act as antioxidants, thus protecting cells and tissues from the damaging
effects of free radicals and singlet oxygen.
Aims: The aim of this study was to find an effective method for carotenoid extraction (Enzymatic
and alkaline treatment) from shrimp waste and compare their antioxidant potential with synthetic
antioxidants butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) using sunflower
Methods: The sunflower oil was exposed to three concentrations of extracted carotenoid (470, 235,
and 118 mg/kg) and synthetic antioxidants. The inhibition of lipid peroxidation was evaluated for
Malondialdehyde and peroxide value.
Results and Discussion: The mean values of carotenoid extract were 243 and 170 mg/kg for enzymatic
and alkaline treatment, respectively. Therefore, the highest efficacy of carotenoid extraction
was obtained from enzymatic extraction. The oil samples containing 470 ppm carotenoid, which
were extracted by alcalase as the default treatment, exhibited a less peroxide value and higher antioxidant
potential than the oil samples containing synthetic antioxidants.
Conclusion: According to the results, the synthetic antioxidants can be replaced by extracted
carotenoids from shrimp waste as a natural antioxidant to inhibit oxidation.