Title:Advances in Sample Digestion Using Microwave-ultraviolet Radiations: Phosphorus and Sulfur Determination in Animal Feed
VOLUME: 17 ISSUE: 4
Author(s):Diogo L. R. Novo, Priscila T. Scaglioni, Rodrigo M. Pereira, Filipe S. Rondan, Gilberto S. Coelho Junior and Marcia F. Mesko*
Affiliation:Centro de Ciencias Quimicas, Farmaceuticas e de Alimentos, Universidade Federal de Pelotas, 96160-000, Capao do Leao, RS, Centro de Ciencias Quimicas, Farmaceuticas e de Alimentos, Universidade Federal de Pelotas, 96160-000, Capao do Leao, RS, Centro de Ciencias Quimicas, Farmaceuticas e de Alimentos, Universidade Federal de Pelotas, 96160-000, Capao do Leao, RS, Centro de Ciencias Quimicas, Farmaceuticas e de Alimentos, Universidade Federal de Pelotas, 96160-000, Capao do Leao, RS, Centro de Ciencias Quimicas, Farmaceuticas e de Alimentos, Universidade Federal de Pelotas, 96160-000, Capao do Leao, RS, Centro de Ciencias Quimicas, Farmaceuticas e de Alimentos, Universidade Federal de Pelotas, 96160-000, Capao do Leao, RS
Keywords:Microwave-assisted ultraviolet digestion, ion chromatography, phosphorus, sulfur, feed safety, minerals determination,
quality control.
Abstract:
Background: Conventional analytical methods for phosphorus and sulfur determination
in several matrices present normally analytical challenges regarding inaccuracy, detectability
and waste generation.
Objective: The main objective is proposing a green and feasible analytical method for phosphorus
and sulfur determination in animal feed.
Methods: Synergic effect between microwave and ultraviolet radiations during sample preparation
was evaluated for the first time for the animal feed digestion associated with further phosphorus
and sulfur determination by ion chromatography with conductivity detection. Dissolved
carbon and residual acidity in the final digests were used for the proposed method assessment.
Phosphorus and sulfur values were compared with those obtained using conventional microwaveassisted
wet digestion in closed vessels associated with inductively coupled plasma optical emission
spectrometry and with those obtained using an official method (Association of Official Analytical
Chemists International). Recovery tests and certified reference material analyses were performed.
Animal feeds were analyzed using the proposed method.
Results: Sample masses of 500 mg were efficiently digested using only 2 mol L-1 HNO3. The results
obtained by the proposed method were not differing significantly (p > 0.05) from those obtained
by conventional and by official methods. Suitable recoveries (from 94 to 99%), agreement
with certified values (101 and 104%), and suitable relative standard deviations (< 8%) were
achieved. Phosphorus and sulfur content in commercial products varied in a wide range (P: 5,873
to 28,387 mg kg-1 and S: 2,165 to 4,501 mg kg-1).
Conclusion: The proposed method is a green, safe, accurate, precise and sensitive alternative for
animal feed quality control.
