Background: Free radicals can lead to liver dysfunction. Quality control of traditional
formulations ensures their safe, pure, and pharmaceutical efficacy. “Qurs-e-Vard”, containing petals
of Rosa damascena Mill., fruits of Rhus coriaria L. and roots of Glycyrrhiza glabra L. has
been suggested as a hepatoprotective preparation in Traditional Persian Medicine (TPM).
Objective: This study was directed at the evaluation of the phytochemical characterization, standardization,
and in vitro antioxidant activity determination of a solid formulation and its components.
Methods: Some qualitative and quantitative controls were performed like ash value, heavy metals
investigation, and microbial contamination. The phytochemical assays were used for obtaining total
phenolic and flavonoid contents with spectrophotometric methods. 2, 2-Diphenyl-1-c (DPPH)
and Nitric Oxide (NO) assays were run for determining Radical scavenging activities of the formulation
and its components. Ferric reducing antioxidant power assay (FRAP) was determined as
Results: Total phenolic contents of hydroalcoholic and aqueous extracts of the polyherbal formulation
measured respectively, (376±0.93) and (297.6±0.96) mg of gallic acid/g of dry matter. Total
flavonoid contents of the formulation were also measured (36.27±0.98) for hydroalcoholic extract
and (17.79±0.86) mg of quercetin/g of dry matter for aqueous extract. The IC50 of hydroalcoholic
and aqueous extract was obtained (88.14±1.15) and (140.78±2.98) μg/ml, respectively. NO scavenging
percentages (200μg/ml) of hydroalcoholic and aqueous extracts were measured
(59.11±2.15) and (65.08±2.35). FRAP values of hydroalcoholic and aqueous extracts were
achieved (255.24±3.45) and (134.57±3.45) μg/ml as well.
Conclusion: Our findings indicated that this polyherbal formulation and its components have justifiable