Background: Breast Cancer (BC) is the most common malignancy among women with a high mortality
rate. The blockade of asparagine-related pathways may be an effective measure to control the progression
and reduction of BC metastasis potential. Recently, it has been shown that various miRNAs, as part of small
non-coding RNAs, have a great role in cancer development, especially asparagine-related pathways, to modulate
Objective: This study aimed to evaluate the expression of miR-130a-5p and miR-615-3p in tumoral and nontumoral
adjacent tissues of patients with BC.
Methods: There is a chance that asparagine metabolism is influenced by miR-130a-5p and miR-615-3p as confirmed
by bioinformatics analysis. Hence, real-time PCR was conducted on eighty BC tumoral and non-tumoral
adjacent tissues to evaluate the expression level of the two miRNAs. To predict the potential biological process
and molecular pathways of miR-130a-5p, an in silico analysis was performed.
Results: This study indicated that miR-130a was downregulated in tumoral tissues compared to non-tumoral
adjacent tissues (P-value= 0.01443 and fold change= -2.5137), while miR-615-3p did not show a significant
difference between the two groups. Furthermore, the subgroup studies did not reveal any significant correlation
between the expression of these two miRNAs and subfactors. Furthermore, in silico studies unraveled several
biological processes related to amino-acid metabolism, as well as pathways related to tumor development such
as Phosphatase and Tensin Homolog (PTEN) and JAK-STAT pathways among miR-130a-5p target genes.
Conclusion: Our findings indicate that miRNA-130a-5p is downregulated in BC tissues and may play a tumor
suppressor role in patients with BC. Therefore, it may be suggested as a potential diagnostic and therapeutic
target for BC.