Abstract
Stimulated emission depletion (STED) microscopy has become a useful tool for visualization and dynamic monitoring at an ultra-high resolution in biological research and material science. For STED technology, fluorescent probes are irreplaceable in the imaging process. Among these probes, organic fluorescent probes have superior photo-stability, high brightness, large Stokes’ shifts and excellent biocompatibility, thus are widely applied in STED microscopy. Based on this consideration, this review presents the recent advances on organic fluorescent probes for STED microscopy, including typical organic fluorescent probes, aggregation-induced emission luminogens (AIEgens), polymer dots, and other nanoparticles. The applications of organic fluorescent probes in biological imaging, such as in live-cell, live-tissue, and in vivo imaging, as well as in material monitoring at the nanoscale using STED microscopy, are also included. This review provides the guidelines for the design of new materials that can be used to enhance the imaging performance of STED microscopy, thus leading to real-world applications.
Keywords: Stimulated emission depletion microscopy, super-resolution imaging, Organic fluorescent probes, biological imaging, material science.
Combinatorial Chemistry & High Throughput Screening
Title:Recent Advances on Organic Fluorescent Probes for Stimulated Emission Depletion (STED) Microscopy
Volume: 24 Issue: 7
Author(s): Ruohan Xu, Yanzi Xu, Zhi Wang, Yu Zhou, Dongfeng Dang*Lingjie Meng*
Affiliation:
- School of Chemistry, MOE Key Laboratory for Non-equilibrium Synthesis and Modulation of Condensed Matter, Xi'an Key Laboratory of Sustainable Energy Material Chemistry, Xi'an Jiao Tong University, Xi'an 710049,China
- School of Chemistry, MOE Key Laboratory for Non-equilibrium Synthesis and Modulation of Condensed Matter, Xi'an Key Laboratory of Sustainable Energy Material Chemistry, Xi'an Jiao Tong University, Xi'an 710049,China
Keywords: Stimulated emission depletion microscopy, super-resolution imaging, Organic fluorescent probes, biological imaging, material science.
Abstract: Stimulated emission depletion (STED) microscopy has become a useful tool for visualization and dynamic monitoring at an ultra-high resolution in biological research and material science. For STED technology, fluorescent probes are irreplaceable in the imaging process. Among these probes, organic fluorescent probes have superior photo-stability, high brightness, large Stokes’ shifts and excellent biocompatibility, thus are widely applied in STED microscopy. Based on this consideration, this review presents the recent advances on organic fluorescent probes for STED microscopy, including typical organic fluorescent probes, aggregation-induced emission luminogens (AIEgens), polymer dots, and other nanoparticles. The applications of organic fluorescent probes in biological imaging, such as in live-cell, live-tissue, and in vivo imaging, as well as in material monitoring at the nanoscale using STED microscopy, are also included. This review provides the guidelines for the design of new materials that can be used to enhance the imaging performance of STED microscopy, thus leading to real-world applications.
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Cite this article as:
Xu Ruohan , Xu Yanzi , Wang Zhi , Zhou Yu , Dang Dongfeng *, Meng Lingjie *, Recent Advances on Organic Fluorescent Probes for Stimulated Emission Depletion (STED) Microscopy, Combinatorial Chemistry & High Throughput Screening 2021; 24 (7) . https://dx.doi.org/10.2174/1386207323666200917104203
DOI https://dx.doi.org/10.2174/1386207323666200917104203 |
Print ISSN 1386-2073 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5402 |
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