A Potential MRI Agent and an Anticancer Drug Encapsulated within CPMV Virus-Like Particles

Title:A Potential MRI Agent and an Anticancer Drug Encapsulated within CPMV Virus-Like Particles

Volume: 24 Issue: 10

Author(s): Alaa A.A. Aljabali*, Lorca Alzoubi, Yassmen Hamzat, Alaa Alqudah, Mohammad A. Obeid, Mazhar S. Al Zoubi, Raed M. Ennab, Walhan Alshaer, Khaled Albatayneh, Bahaa Al-Trad, Dana A. Alqudah, Dinesh Kumar Chellappan, Gaurav Gupta, Murtaza M. Tambuwala, Dua Kamal and David J. Evans

Affiliation:

• Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Yarmouk University, Irbid,Jordan

Keywords: Virus-like particles, viruses, bioconjugation, drug delivery, cisplatin, nanomedicine.

Abstract:

Background: Virus nanoparticles have been extensively studied over the past decades for theranostics applications. Viruses are well-characterized, naturally occurring nanoparticles that can be produced in high quantity with a high degree of similarity in both structure and composition.

Objectives: The plant virus Cowpea Mosaic Virus (CPMV) has been innovatively used as a nanoscaffold. Utilization of the internal cavity of empty Virus-Like Particles (VLPs) for the inclusion of therapeutics within the capsid has opened many opportunities in drug delivery and imaging applications.

Methods: The encapsidation of magnetic materials and anticancer drugs was achieved. ^SuperscriptCPMV denotes molecules attached to the external surface of CPMV and CPMV_Subscript denotes molecules within the interior of the capsid.

Results: Here, the generation of novel VLPs incorporating iron-platinum nanoparticles _TCPMV_FePt and cisplatin (Cis) (_TCPMV_Cis) is reported. _TCPMV_Cis exhibited a cytotoxic IC₅₀ of _TCPMV_Cis on both A549 and MDA-MB-231 cell lines of 1.8 μM and 3.9 μM, respectively after 72 hours of incubation. The _TCPMV_FePt were prepared as potential MRI contrast agents.

Conclusion: Cisplatin loaded VLP (_TCPMV_Cis) is shown to enhance cisplatin cytotoxicity in cancer cell lines with its potency increased by 2.3-folds.

Cite this article as:

Aljabali A.A. Alaa *, Alzoubi Lorca , Hamzat Yassmen , Alqudah Alaa , Obeid A. Mohammad , Al Zoubi S. Mazhar , Ennab M. Raed , Alshaer Walhan , Albatayneh Khaled, Al-Trad Bahaa, Alqudah A. Dana, Chellappan Kumar Dinesh, Gupta Gaurav , Tambuwala M. Murtaza, Kamal Dua and Evans J. David, A Potential MRI Agent and an Anticancer Drug Encapsulated within CPMV Virus-Like Particles, Combinatorial Chemistry & High Throughput Screening 2021; 24 (10) . https://dx.doi.org/10.2174/1386207323666200914110012