Background: Virus nanoparticles have been extensively studied over the past decades
for theranostics applications. Viruses are well-characterized, naturally occurring nanoparticles that
can be produced in high quantity with a high degree of similarity in both structure and composition.
Objectives: The plant virus Cowpea Mosaic Virus (CPMV) has been innovatively used as a nanoscaffold.
Utilization of the internal cavity of empty Virus-Like Particles (VLPs) for the inclusion of
therapeutics within the capsid has opened many opportunities in drug delivery and imaging applications.
Methods: The encapsidation of magnetic materials and anticancer drugs was achieved. SuperscriptCPMV
denotes molecules attached to the external surface of CPMV and CPMVSubscript denotes
molecules within the interior of the capsid.
Results: Here, the generation of novel VLPs incorporating iron-platinum nanoparticles TCPMVFePt
and cisplatin (Cis) (TCPMVCis) is reported. TCPMVCis exhibited a cytotoxic IC50 of TCPMVCis on
both A549 and MDA-MB-231 cell lines of 1.8 μM and 3.9 μM, respectively after 72 hours of incubation.
The TCPMVFePt were prepared as potential MRI contrast agents.
Conclusion: Cisplatin loaded VLP (TCPMVCis) is shown to enhance cisplatin cytotoxicity in cancer
cell lines with its potency increased by 2.3-folds.