Background: Boswellic acids are the main constituents of Boswellia serrata gum. These comprises of four pentacyclic triterpenes, 11-keto-β-boswellic acid (KBA) being one of them.
Objectives: Comparing the extraction efficiency of KBA through microwave-assisted extraction (MAE) and ultrasoundassisted extraction (UAE) followed by optimizing the extraction process using response surface methodology (RSM) and
validation of HPTLC.
Methods: UAE and MAE of KBA were carried out employing methanol as extracting solvent. Working out the better mode
of extraction, single factorial experiments were conducted for further optimization. Design expert software was used for optimization purpose where solvent to drug ratio, extraction temperature and extraction time were taken as input variables.
Quantification of KBA in each extract was done through High Performance Thin Layer Chromatography (HPTLC) and the
method was validated as per International Council for Harmonization (ICH) guidelines.
Results: UAE stood out to be better mode of extraction for KBA. Solvent to drug ratio of 21.54 mL/gram, extraction temperature of 45.12°C and extraction time of 10.02 minutes were established as optimum conditions which yielded 8.59%w/w
of KBA. Regarding HPTLC, the Rf value of KBA was measured and correlation coefficient was calculated from standard
curve. Accuracy, precision and recovery were found within limits.
Conclusion: From this study, it was concluded that a non-thermal method is better choice of extraction for KBA. All the input variables significantly affected KBA content which was confirmed by model fitting. Moreover, HPTLC method was
developed for quantification of KBA which was found to be accurate, reliable and highly sensitive.