Objective: Madhuca longifolia has been used for the treatment of renal cancer. Therefore,
the current study describes the protective effects of biofabricated silver nanoparticles (MLAg-
NPs) using Madhuca longifolia aqueous leaves extract against diethylnitrosamine (DEN) induced
Renal Cell Carcinoma (RCC) in rats.
Methods: Animals were categorized into five groups and treated with doses of silver nanoparticles
for 16 weeks. Antineoplastic effect in renal cancer was dose dependent to control the macroscopical
variations when compared to DEN induced group. Significant changes were observed in biochemical
parameters and dose graded improvement in the level of antioxidants parameters were accountable
for its protective nature.
Results: Silver nanoparticles in dose dependent manner was effective to modify the raised levels of
pro-inflammatory cytokines and inflammatory mediators during renal cancer. Alteration in renal
histopathology were also detected in the silver nanoparticles treated group, which show its safety
concern. Biofabricated silver nanoparticles (MLAgNPs) using Madhuca longifolia can convey significant
chemo-protective effect against renal cancer by suppressing the IL-6, TNF-α and IL-1β by
nuclear factor-kappa B (NF-κB) pathway.
Conclusion: Our outcomes implicates that biofabricated MLAgNPs exhibited a chemoprotective
potential in the prevention and intervention of RCC.