Metabolic Analysis of Triptolide Microspheres in Human, Dog, Rabbit and Rat Liver Microsomes with UPLC-MS/MS Method

Title:Metabolic Analysis of Triptolide Microspheres in Human, Dog, Rabbit and Rat Liver Microsomes with UPLC-MS/MS Method

Volume: 17 Issue: 9

Author(s): Li Juan Wang*, Yan Liu, Rui Li and Dong Xian He

Affiliation:

• Pharmacy College, Chongqing Engineering Research Center of Pharmaceutical Sciences, Chongqing Medical and Pharmaceutical College, Chongqing,China

Keywords: Triptolide, UPLC-MS/MS, high-resolution MS, liver microsomes, microspheres, metabolic analysis.

Abstract:

Objectives: Riptolide (TPL) has been shown to have a good clinical effect on rheumatoid arthritis (RA). We designed TPL microspheres (TPL-MS) and investigated its metabolic behavior in human, dog, rabbit and rat liver microsomes (HLM, DLM, RLM and SDRLM) with UPLC- MS/MS method.

Methods: First, a UPLC-MS/MS method was established to measure the concentration of TPL in samples. The sample was separated on a C18 column (2.1×100 mm, 1.8μm) and eluted with a gradient elution. The precursor ions/product ions were m/z 378.1/361.0 for TPL and 260.0/116.2 for the internal standard. Then T_1/2, V_max and CL_int were calculated from the above data. Finally, the metabolites of TPL-MS were identified by high-resolution UPLC-MS/MS. The sample was separated on a C18 column (2.1×100 mm, 2.2 μm) and eluted with isocratic elution. Mass spectrometric detection was carried out on a thermo Q-exactive mass spectrometer with HESI. The scanning range of precursor ions was from m/z 50 to m/z 750.

Results: Through several indicators including standard curve, precision, accuracy, stability, matrix effect and recovery rate, the enzymatic kinetics parameters including T_1/2, V_max and CL_int were evaluated. Several metabolites of TPL-MS were identified.

Discussion: UPLC-MS/MS method is an accurate and sensitive method for the determination of TPL in liver microsome samples with good precision, accuracy and stability. The variation of parameters indicated that the microspheres can delay the elimination of TPL in liver microsomes.

Conclusion: The metabolism of TPL-MS varied among species, but no new metabolites appeared.

Cite this article as:

Wang Juan Li *, Liu Yan , Li Rui and He Dong Xian , Metabolic Analysis of Triptolide Microspheres in Human, Dog, Rabbit and Rat Liver Microsomes with UPLC-MS/MS Method, Current Pharmaceutical Analysis 2021; 17 (9) . https://dx.doi.org/10.2174/1573412917999200909143213