MicroRNAs Profiling in HIV, HCV, and HIV/HCV Co-Infected Patients

Author(s): Mohsen Moghoofei, Sohrab Najafipour, Shayan Mostafaei, Ahmad Tavakoli, Farah Bokharaei-Salim, Saied Ghorbani, Davod Javanmard, Hadi Ghaffari, Seyed Hamidreza Monavari*

Journal Name: Current HIV Research

Volume 19 , Issue 1 , 2021


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Abstract:

Background: Human immunodeficiency virus (HIV) and hepatitis C virus (HCV) infections are important public health issues.

Objective: This study aimed to assess the association between microRNAs expression leveland immunological and viral markers in HIV, HCV, and HIV/HCV co-infected patients.

Methods: The expression level of miR-29, miR-149, miR-199, miR-let7, miR-223, miR-155, miR-122, and miR-150 was evaluated in 20 HIV, 20 HCV, 20 co-infected patients, and 20 healthy controls using real-time PCR assay. HIV and HCVviral loads were measuredby real-time PCR, and also, CD4+ T-lymphocyte count was measuredby the PIMA CD4 analyzer.

Results: The miRNA expression pattern in each mentioned group showed significantly different expression profiles, but some miRNA species were shared between the groups. MiR-122 and miR-155 were upregulated, while miR-29 and miR-223 were downregulated in three patients groups compared to healthy controls. A significant positive correlation was observed between the expression of miR-122 and HIV/HCV loads. But, miR-29 and let-7 were negatively correlated with HIV load, and miR-149 and let-7 were negatively correlated with HCV load. Also, miR-155 was positively correlated with HCV load. MiR-122 and miR-199 were negative while others were positively correlated with CD4+ T cell count.

Conclusion: These miRNAs are probably involved in the clinical progression and pathogenesis of HIV and HCV infections. Therefore, determining and manipulating these miRNAs can lead to opening a new gate to control these important infections.

Keywords: HIV, HCV, coinfection, microRNA, PBMC, AIDS.

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Article Details

VOLUME: 19
ISSUE: 1
Year: 2021
Published on: 26 January, 2021
Page: [27 - 34]
Pages: 8
DOI: 10.2174/1570162X18666200908112113
Price: $65

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