Effect of pH and Temperature on the Growth and Laccases Production in the Cultivation of Pleurotus sajor-caju PS-2001 in Stirred-tank Bioreactor

Author(s): Fernanda Bettin*, Letícia O. da Rosa, Queli Montanari, Aldo J. P. Dillon, Mauricio M. da Silveira

Journal Name: Current Biotechnology

Volume 9 , Issue 3 , 2020


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Abstract:

Background: Laccases are multi-copper enzymes that oxidize phenolic/aromatic compounds and represent a promising alternative to environmental decontamination processes and biotechnological applications.

Objective: The effects of pH and temperature on the growth and the production of laccases during the cultivation of Pleurotus sajor-caju PS-2001 in stirred-tank bioreactor were assessed.

Methods: Assays were performed at fixed pH values from 4.5 to 7.5 (28°C) and at temperatures from 24 to 36°C (pH 6.5).

Results: In pH testing, larger biomass concentration (4.5 g L-1) was reached at pH 5.5, whereas concentrations of 3.7, 3.1 and 1.7 g L-1 were measured at pH 4.5, 6.5 and 7.5, respectively. With ABTS as substrate, peaks of laccases activity of 50, 30 and 24 U mL-1, at pH 6.5, 5.5 and 7.5, respectively, were detected. Under different temperatures, higher mycelial concentrations (3.0 g L-1) were quantified at 66 hours at 28°C, while concentrations below 2.0 g L-1 were observed at 24, 32, and 36°C. Maximum laccases activities of 50, 42, 6 and 5 U mL-1 were obtained at 28, 32, 24, and 36°C, respectively. In all tests, the presence of other phenol oxidases – total peroxidase, manganese peroxidase, lignin peroxidase and veratryl alcohol oxidase – was observed.

Conclusion: The results indicate that variations in pH and temperature during fungal cultivation strongly affect the enzymatic activity and growth kinetics of P. sajor-caju PS-2001 in a stirredtank bioreactor.

Keywords: Pleurotus sajor-caju, laccases, phenol oxidases, pH, temperature, submerged process, growth kinetics, stirred-tank bioreactor.

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Article Details

VOLUME: 9
ISSUE: 3
Year: 2020
Published on: 21 December, 2020
Page: [219 - 229]
Pages: 11
DOI: 10.2174/2211550109999200908090053
Price: $65

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