Background: The key ingredients of e-cigarettes liquid are commonly propane-1,2-diol (also called propylene
glycol) and propane-1,2,3-triol (vegetal glycerol) and their antimicrobial effects are already established. The nicotine and
flavors which are often present in e-liquids can interfere with the growth of some microorganisms. Objective: The effect of
the combining these elements in e-liquids is unknown. The aim of the study was to investigate the possible effects of these
liquids on bacterial growth in the presence or absence of nicotine and flavors.
Methods: Susceptibilities of pathogenic strains (Klebsiella pneumoniae, Staphylococcus aureus, Pseudomonas aeruginosa,
Acinetobacter baumannii, Escherichia coli, Enterococcus faecalis and Sarcina lutea) were studied by means of a multidisciplinary approach. Cell viability and antioxidant assays were also evaluated.
Results: All e-liquids investigated showed antibacterial activity against at least one pathogenic strain. A higher activity was
correlated to the presence of flavors and nicotine.
Discussion: In most cases the value of minimal bactericidal concentration is equal to the value of minimal inhibitory concentration showing that these substances have a bactericidal effect. This effect was observed in concentrations up to 6.25%
v/v. Antioxidant activity was also correlated to presence of flavors. Over time, the viability assay in human epithelial lung
A549 cells showed a dose-dependent inhibition of cell growth.
Conclusion: Our results have shown that flavors considerably enhance the antibacterial activity of propane-1,2-diol and
propane-1,2,3-triol. This study provides important evidence that should be taken into consideration in further investigative
approaches, to clarify the different sensitivity of the various bacterial species to e-liquids, including the respiratory microbiota, to highlight the possible role of flavors and nicotine.