Aim: The aim of the study was to formulate, characterize, and evaluate the Resveratrol-
loaded Cubosomes (RC) for topical application.
Background: Resveratrol (RV) is a nutraceutical compound with exciting pharmacological potential
in different diseases, including cancers. Many studies on resveratrol have been reported for anti-
melanoma activity. Due to its low bioavailability, the therapeutic activities of resveratrol are
strongly limited. Hence, an approach with nanotechnology has been made to increase its activity
through transdermal drug delivery.
Objective: To formulate, characterize, and evaluate the resveratrol-loaded cubosomes (RC). To
evaluate Resveratrol-loaded Cubosomal Gel (RC-Gel) for its topical application.
Methods: RC was formulated by homogenization technique and optimized using a 2-factor 3-level
factorial design. Formulated RCs were characterized for particle size, zeta potential, and entrapment
efficiency. Optimized RC was evaluated for in vitro release and stability study. Optimized
RC was further formulated into cubosomal gel (RC-Gel) using carbopol and evaluated for drug
permeation and deposition. Furthermore, developed RC-Gel was evaluated for its topical application
using skin irritancy, toxicity, and in vivo local bioavailability studies.
Results: The optimized RC indicated cubic-shaped structure with mean particle size, entrapment efficiency,
and zeta potential were 113±2.36 nm, 85.07 ± 0.91%, and -27.40 ± 1.40 mV, respectively.
In vitro drug release of optimized RC demonstrated biphasic drug release with the diffusion-controlled
release of resveratrol (RV) (87.20 ± 3.91%). The RC-Gel demonstrated better drug permeation
and deposition in mice skin layers. The composition of RC-Gel has been proved non-irritant to
mice skin. In vivo local bioavailability study depicted the good potential of RC-Gel for skin localization.
Conclusion: The RC nanoformulation proposes a promising drug delivery system for melanoma
treatment simply through topical application.