Background: Citrus limon a small evergreen plant belongs to the family Rutaceae. These
species are extensively cultivated throughout the world because of their multiple health benefits
for humans and their applications in the pharmaceutical and food industries. Various studies
were conducted using their plant parts (fruits, flowers, peels, leaves, blossoms) but the studies on
peel extracts are very limited. However, the anticonvulsant activity of peels has not been studied
Objective: The main goal of this study is to appraise the anticonvulsant effect stimulated by the antioxidant
property of hydroalcoholic extracts of Citrus limon (HAECL) peels in various animal
Methods: The anticonvulsant and in vivo antioxidant activity of HAECL peels was observed by
Maximal electric shock (MES) model, pentylenetetrazole (PTZ) induced clonic convulsion model
and PTZ induced kindling test. The extract was administered to test groups at doses of 200, 400
and 600 mg/kg. orally in PTZ and MES methods. The highest dose of extract was given to the test
grouped animals in case of a kindling test. After completion of the time period of kindling, the
brains of all grouped animals were isolated and subjected to analyse oxidative stress parameters
such as malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione
(GSH) biochemically to investigate the antioxidant profile of the plant.
Results: HAECL peels at doses of 400 and 600 mg/kg significantly (p<0.01) delayed the onset, decreased
the duration of myoclonic spasm in PTZ induced seizure model and also significantly
(p<0.01) decreased the duration of hind limb tonic extension (HLTE) as well as significantly
(p<0.05) increased the postictal depression (PID) in MES model compared to control. In the PTZinduced
kindling model, the malondialdehyde (MDA) level was elevated with a diminished level
of SOD, CAT, GSH compared to the control group but pretreatment with HAECL at the highest
dose reduced the MDA level and refined SOD, CAT and GSH status effectively.
Conclusion: From the above investigation, it was concluded that HAECL could produce significant
anticonvulsant activity and also attenuate oxidative stress-induced during a seizure.